Network Formation Assay Data

Neurotoxicity test using a network formation assay studying the inhibition of network formation at acrylamide exposure.

Usage

data(nfa)

Format

A data frame with 45 observations on the following 4 variables.

chip: chip ID
conc: 7 concentrations of acrylamide, ranging from 0-5mM
experiment: factor with levels 1 or 2 denoting two consecutive experiments
response: Number of connections [%]

References

Frimat, JP, Sisnaiske, J, Subbiah, S, Menne, H, Godoy, P, Lampen, P, Leist, M, Franzke, J, Hengstler, JG, van Thriel, C, West, J. The network formation assay: a spatially standardized neurite outgrowth analytical display for neurotoxicity screening. Lab Chip 2010; 10:701-709.

Examples

data(nfa)

## Fit a four-parameter log-logistic model
nfa.m1 <- drm(response ~ conc, data = nfa, fct = LL.4())
summary(nfa.m1)
#> 
#> Model fitted: Log-logistic (ED50 as parameter) (4 parms)
#> 
#> Parameter estimates:
#> 
#>                Estimate Std. Error t-value   p-value    
#> b:(Intercept)  1.828165   0.429684  4.2547 0.0001184 ***
#> c:(Intercept) -3.879086   5.620773 -0.6901 0.4939982    
#> d:(Intercept) 88.533870   2.366218 37.4158 < 2.2e-16 ***
#> e:(Intercept)  0.730916   0.086889  8.4121 1.813e-10 ***
#> ---
#> Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
#> 
#> Residual standard error:
#> 
#>  9.591243 (41 degrees of freedom)
plot(nfa.m1, main = "NFA dose-response")